Books & Book Chapters

Book Author: Immunohistochemistry. Essential Elements and Beyond. Springer, 2016.

Book Chapters:

  1. Hopp N, Hagen J, Aggeler B and A Kalyuzhny (2017) Express γ-H2AX Immunocytochemical Detection of DNA Damage. In: Fast Detection of DNA Damage. Methods Mol Biol. Vol.1664:123-128.
  2. Hopp N, Hagen J, Aggeler B and A. Kalyuzhny (2017) Automated High-Content Screening of γ-H2AX Expression in HeLa Cells. In: Signal Transduction Immunohistochemistry 2nd Edition. Methods Mol Biol. Vol.1554:273-283.
  3. Schwartz D, Grahek M, He Y, Wang W, Nguyen J and A Kalyuzhny (2017) Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry. In: Signal Transduction Immunohistochemistry 2nd Edition. Methods Mol Biol. Vol.1554:219-227.
  4. Grahek M, Ptak A and A. Kalyuzhny (2017) High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues Using Secondary Antibody Conjugated to Polymer-HRP. In: Signal Transduction Immunohistochemistry 2nd Edition. Methods Mol Biol. Vol.1554:211-218.
  5. Hagen J, Schwartz D, and A. Kalyuzhny (2017) Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species. In: Signal Transduction Immunohistochemistry 2nd Edition. Methods Mol Biol. Vol.1554:155-160.
  6. Kalyuzhny A. (2015) Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays. In: Western Blotting. Methods Mol Biol. Vol.1312:435-437. 
  7. Jodi Hagen, JP Houchins and Alexander Kalyuzhny (2012) ELISpot assay as a tool to study oxidative stress in lymphocytes. In: Handbook of Elispot 2nd Edition. Methods Mol Biol. Vol.792:87-96.
  8. Jodi Hagen, JP Houchins and Alexander Kalyuzhny (2012) Combining ELISpot and ELISA to measure amounts of cytokines secreted by a single cell. In: Handbook of Elispot 2nd Edition. Methods Mol Biol. Vol. 792:115-122.
  9. Jodi Hagen, JP Houchins and Alexander Kalyuzhny (2012) ELISpot assay for neuroscience research: studying TNF-alpha secretion from microglial cells. In: Handbook of Elispot 2nd Edition. Methods Mol Biol. Vol. 792:97-104.
  10. Jodi Hagen, Chris Hartnett, JP Houchins, Steeve Giguѐre and Alexander Kalyuzhny (2012) Equine ELISpot assay to study secretion of IFN-gamma and IL-4 from peripheral blood mononuclear cells. In: Handbook of Elispot 2nd Edition. Methods Mol Biol. Vol. 792: 39-45.
  11. Jordan Schoephoerster, Jillian Frisch, Michael Grahek, Chun Wu, Yingwei He, Wei Wang, Jennifer Nguyen, David Schwartz and Alexander Kalyuzhny (2011) Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads. In: Signal Transduction Immunohistochemistry. Methods Mol Biol.Vol 717:291-300.
  12. Jillian Frisch, J.P. Houchins, Michael Grahek, Jordan Schoephoerster, Jodi Hagen, Joseph Sweet, Leopoldo Mendoza, David Schwartz and Alexander Kalyuzhny (2011) Novel multi-color immunofluorescence technique using primary antibodies raised in the same host species. In: Signal Transduction Immunohistochemistry. Methods Mol Biol.Vol 717:233-244.
  13. Hagen J, Houchins J.P, Kalyuzhny A.E. (2011) ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells. In: Methods Mol Biol. Vol. 682:207-215.
  14. Kalyuzhny A.E. (2011) Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage. In: Methods Mol Biol. Vol. 682:15-27.
  15. Kalyuzhny A.E. (2009) ELISPOT assay on membrane microplates. In: Methods Mol Biol. Vol. 536:355-365.
  16. Palzer S, Bailey T, Hartnett C, Grant A, Tsang M, Kalyuzhny A.E. (2005) Simultaneous detection of multiple cytokines in ELISPOT assays. In: Methods Mol Biol. Vol 302:273-88.
  17. Kalyuzhny A.E. (2005) Chemistry and biology of the ELISPOT assay (Review). In:Methods Mol Biol. Vol. 302:15-31.
  18. Grant A, Palzer S, Hartnett C, Bailey T, Tsang M, Kalyuzhny A.E. (2005) A cell-detachment solution can reduce background staining in the ELISPOT assay.  In: Methods Mol Biol. Vol. 302:87-94
  19. Kalyuzhny, A.E. (2003) Immunohistochemical localization of mu-, delta- and kappa-opioid receptors in neurons within antinociceptive brainstem circuits. In: Methods in Molecular Medicine. Opioid Research: Methods and Protocols. Humana Press, Vol. 84: 79-93.
  20. Kalyuzhny, A.E. (2002) Simultaneous in situ detection of DNA fragmentation and RNA/DNA oxidative damage using TUNEL assay and immunohistochemical labeling for 8-hydroxy-2’-deoxyguanosine (8-OHdG). in: Methods in Apoptosis Research. Humana Press, Vol. 203:219-234.
  21. Kalyuzhny, A.E., Elde, R., Loh, H.H. (1995) A method for monitoring the extent of silver intensification of colloidal gold staining".  in: Immunogold silver staining. Methods and applications (ed. M.A. Hayat). Academic Press pp 47-56.
  22. Kalyuzhny, A.E., Mironova, O.V., Platonov, E.S., Sukhikh, G.T. (1990) Effects of opioids on  development of preimplantation mouse embryos. In: Proceedings  of  the  7th  Reiner  de  Graaf  symposium  "From   ovulation to implantation" Elsevier  (Eds J.L.H.Evers & M.J.Heineman), 231-236.